EPCO-30. IDENTIFICATION OF PRECANCEROUS CELLS LEADING TO INTRATUMORAL HETEROGENEITY IN GLIOBLASTOMA

Abstract Glioblastoma (GBM) is the most common and aggressive primary brain cancer in adults. Although multiple oncogenic mutations have been identified neural stem cells of subventricular zone (SVZ) humans, dynamic process how tumor initiation begins with a mutation-harboring remains elusive. To better understand GBM evolves, we analyzed longitudinal transcriptomic changes using spontaneous, somatic animal model EGFRvIII, TP53, PTEN mutations. Using single-cell RNA sequencing, precancerous cell populations originating from oligodendrocyte precursor cells, increased proliferation decreased capacity to differentiate into oligodendrocytes. The undergo malignant transformation become heterogeneous characterized by immune evasion, mesenchymal transition, cycle. We further SVZ human patients chromosomal aberrations. Our findings suggest that evolution closely related specification, time-dependent novel therapeutic target for evolution.